NRL Cell cultures and rickettsia
Head of Laboratory
Assoc. Prof.
Petya Genova-Kalou, PhD
1233 Sofia, Bulgaria; 44A General Stoletov blvd.
tel. 02/9313818; 02/9313817
email:
Laboratory staff:
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Ekaterina Raycheva Mihailova - senior medical laboratory assistant
-
Katya Atanasova Georgieva-Dimitrova - laboratory assistant
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Dr. Snezhana Dikova – trainee doctor
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Dragana Angelov - trainee biologist
-
Nina Kamenova - laboratory worker
History of laboratory
Laboratory "Cell cultures, rickettsia and oncogenic viruses" was established in 2019 by the merger of NRL "Herpes and oncogenic viruses" and NRL "Rickettsiae and cell cultures".
The laboratory carries out diagnostic, research, educational and organizational-methodological activities in the field of herpes and papilloma virus infections, rickettsia and chlamydia infections.
Diagnostic activity
In the NRL "Cell cultures, rickettsia and oncogenic viruses" diagnostic tests are performed dignostic tests for:
- Herpes simplex virus type 1 (HSV 1)
- Herpes simplex virus type 2 (HSV 2)
- Varicella/Herpes zoster virus (VZV / HZV)
- Cytomegalovirus (CMV)
- Epstein-Barr virus (EBV)
- Human herpes virus type 6 (HHV-6)
- Coxiella burnetii
- Rickettsia conorii
- Mycoplasma pneumoniae
- Chlamydophila pneumoniae
- Chlamydia psittaci
- Chlamydia trachomatis
Diagnosis is performed by classical and contemporary molecular-biology techniques (ELISA, PCR and IFA).
↓ Proposed tests depending on the infection ↓
Virus | Clinic | Appropriate study/clinical materials/ | Applied tests |
---|---|---|---|
Herpes simplex virus (HSV) type 1 and type 2 | Labial and genital herpes, gingivostomatitis; ocular pathology, neuropathology - encephalitis, meningitis, myelitis, etc., pneumonia, hepatitis, esophagitis, etc., neonatal herpes | Serum, whole blood, materials from skin and genital lesions, eye secretions, urethral and vaginal secretions, cerebrospinal fluid, biopsy tissue, etc. |
ELISA – detection of IgG and IgM antibodies to HSV-1 and HSV-2 infections PCR - detection of HSV-1 / HSV-2 DNA |
Varicella / Herpes zoster virus (VZV/HZV) | Varicella, herpes zoster, ocular pathology, neuropathology, congenital varicella syndrome, perinatal varicella | Serum, skin rash, eye secretions, cerebrospinal fluid, amniotic fluid, biopsy tissue, etc. |
ELISA – detection of IgG and IgM antibodies to VZV/ HZV PCR - detection of VZV / HZV DNA |
Cytomegalovirus (CMV) | Mononucleosis syndrome, neuropathology, ocular pathology, hepatitis, esophageal and gastrointestinal ulcerations, congenital CMV infection | Serum, whole blood, cerebrospinal fluid, eye secretions, urine, amniotic fluid, biopsy tissue, etc. |
ELISA – detection of IgG and IgM antibodies to CMV PCR - detection of CMV DNA |
Epstein-Barr virus (EBV) | Infectious mononucleosis, neuropathology, hepatitis, nasopharyngeal carcinoma, lymphoproliferative diseases | Serum, whole blood, cerebrospinal fluid, biopsy tissue, etc. |
ELISA - detection of antibodies to EBV: VCA IgG, VCA IgM, EA IgG, EA IgM and EBNA PCR - detection of EBV DNA |
Human herpes virus type 6 (HHV-6) | Exanthema subitum (roseola infantum), neuropathology, lymphoproliferative diseases, multiple sclerosis, chronic fatigue syndrome | Serum, whole blood, cerebrospinal fluid, biopsy tissue, etc. | PCR - detection of HHV-6 DNA |
Coxiella burnetii Q fever |
Acute form of Q fever Chronic form of Q fever |
Serum, whole blood, plasma, biopsy tissue, bronchoalveolar lavage, urine, removed heart valves and prostheses, pleural puncture, ticks in all stages of development |
ELISA - detection of IgG and IgM phase II and phase I antibodies to Coxiella burnetii; IFA - determination of IgG and IgM phase II and phase I antibodies to Coxiella burnetii PCR - detection of Coxiella burnetii DNA |
Rickettsia conorii | Marseille fever | Serum, whole blood, plasma, biopsy tissue, skin rash, ticks in all stages of development | ELISA – detection of IgG and IgM antibodies to Rickettsia conorii |
Mycoplasma pneumoniae | Atypical pneumonia, neuropathology - encephalitis, meningitis, cardiovascular complications - pericarditis, myocarditis, skin complications - erythematous, maculopopular and bullous rashes, exanthemas, arthropathies | Serum, whole blood, plasma, bronchoalveolar lavage, throat secretions, sputum, biopsy tissue, etc |
ELISA – detection of IgG and IgM antibodies to Mycoplasma pneumoniae PCR - detection of Mycoplasma pneumoniae DNA |
Chlamydophila pneumoniae | Atypical pneumonia, neuropathology - encephalitis, meningitis, cardiovascular complications - pericarditis, myocarditis, skin complications - erythematous | Serum, whole blood, plasma, biopsy tissue, bronchoalveolar lavage, nasopharyngeal secretions, sputum, etc. |
ELISA – detection of IgG and IgM antibodies to Chlamydophila pneumoniae PCR - detection of Chlamydophila pneumoniae DNA |
NRL "Cell cultures, rickettsiae and oncogenic viruses" is accredited according to BDS / ISO 17025: 2006 as Sector "Rickettsia and Chlamydia" and Sector "Herpes viruses" of LIC of NCIPD. Every year the quality of the diagnostic work in the laboratory is controlled and certified by the Institute for Standardization and Documentation of Medical Laboratories Düsseldorf, Germany.
The laboratory has a collection of over 50 different types of human and animal cell lines, necessary for the routine diagnosis of NRL "Enteroviruses" and NRL "Influenza and ARD", as well as for the implementation of research activities.
Organizational and methodological activity
In its capacity of NRL "Cell cultures, rickettsia and oncogenic viruses" organizes and conducts twice a year quality control of diagnostic work of virological laboratories in the country.
The laboratory maintains contact and cooperates with the microbiological laboratories of RHI, MHAT, DCC and private microbiological and virological laboratories, the microbiological laboratories of infectious diseases hospitals in the country, the departments of microbiology and virology of medical universities in Sofia, Varna, Plovdiv, Pleven and Stara Zagora. and with the Laboratory of Rickettsia and Chlamydia Infections at the Bulgarian Food Safety Agency (BFSA), National Diagnostic Research Veterinary Medical Institute, Sofia.
Educational activity
The laboratory conducts specialized courses under the auspices of SDO for the acquisition of the medical specialty "Virology" of doctors and biologists. It is organized annually outside the optional training of students, postgraduates and doctoral students, as well as undergraduate internship and preparation of diploma theses of masters in various specialties.
In the laboratory three diploma theses were successfully made and defended by students of the Faculty of Biology of Sofia University “St. Kliment Ohridski ”and two students from UCTM - Sofia for obtaining bachelor’s and master’s degrees. There is currently one full-time doctoral student in virology in the laboratory.
Scientific activity
The laboratory works and annually presents research projects for funding from the Research Fund of the Ministry of Education and Science. Participates in the implementation of national and international projects.
The laboratory specializes in cytotoxicity testing, as well as in performing anti-viral testing of newly synthesized substances and natural products in vitro.
Regularly publishes the results of his work in specialized international and Bulgarian scientific journals and reports at national and international scientific forums.
Participation in projects:
- Research project on the topic: "Study of the chemical composition, antiviral and antibacterial activity of extracts from the plant Graptopetalum paraguayense E. Walter (Crassulaceae)"
“Scientific Research Fund” Competition for financing scientific research - 2017, Contract № DN19 / 16, 20.12.2017
Head of the partner organization: Assoc. Prof. Petia Genova-Kalou, PhD.
erm of implementation: 2018 - 2020. - Research project on the topic: "Molecular genetic identification and creation of an archival genomic bank of circulating human and animal C. burnetii genotypes and determining their role as particularly dangerous infectious agents causing epizootic and epidemiological outbreaks in the Republic of Bulgaria”
"Scientific Research Fund" Competition for research funding - 2019
Supervisor: Assoc. Prof. Petia Genova-Kalou, PhD.
Term of implementation: 2019 - 2022.
Important publications:
- Genova-Kalou P., Vladimirova N., Stoitsova S., Krumova S., Kurchatova A., Kantardjiev T. (2019). Q fever in Bulgaria: laboratory and epidemiological findings on human cases and outbreaks, 2011 to 2017. Euro Surveill., 24(37): pii = 1900119. https://doi.org/10.2807/1560-7917 (IF = 7.4)
- Zaharieva M., Genova-Kalou P., Dincheva I., Badjakov I., Krumova S., Enchev V., Najdenski H., Markova N. (2019). Anti-Herpes Simplex virus and antibacterial activities of Graptopetalum paraguayense E. Walther leaf extract: a pilot study. Biotechnology and Biotechnological Equipment, 33(1): 1251 – 1259. doi: 10.1080/13102818.2019.1656108 (IF = 1.097)
- Krumova St., Pavlova A., Yotovska K., Genova-Kalou P. (2019). Combined laboratory approach to detection of Parvovirus B19 and Coxiella burnetii in patients with fever of unknown origin. Clinical Laboratory, 65: 69 – 76. (IF = 0.955)
- Ivanova Stefka, Petia Genova-Kalou, Daniela Pencheva, Zafira Mihneva. A combined serological and molecular approach to the laboratory testing of patients with reactive arthritis - detection of parvovirus B19 and Chlamydia trachomatis. Comp. Ren.de l’Acad. Bulg. Des. Sci., 2016, 69 (2), 197 - 202. (IF = 0.284)
- Stefka Kr. Ivanova, Petia D. Genova-Kalou, Silvia Em. Voleva, Svetla G. Angelova, Borislav M. Marinov, Viktor Em. Manolov, Stoian Shishkov. Infectious Agents and Miscarriage in Bulgaria. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS), 2016, 25(1), 1 - 10. (IF = 0.296)
- Voleva S., Ivanova S., Marinov B., P. Genova-Kalou, V. Manolov, V. Vasilev. New approach in the diagnostic algorithm of infectious agents (parvovirus B19 and Chlamydia trachomatis) involved in the development of pathological pregnancy. Obstetrics and Gynecology, 2016, 55(3), 3 – 10.
- Adelina P. Pavlova, Stefka Kr. Ivanova, Petia D. Genova-Kalou. The role of some viral and rickettsial agents in the development of unclear febrile illness accompanied by a rash. Medical Magazine, 2016, 35, 38 - 44.
- Pencheva, D., Genova-Kalou, P., Bryaskova, R. Comparative study of methods for inactivation of vaccines in development process of veterinary “ghost” vaccine. Comptes Rendus de L'Academie Bulgare des Sciences, 2016, 69(5), 675 - 682. (IF = 0.284)
- Daniela Pencheva, Tzvetan Velinov, Petia Genova-Kalou, Todor Kantardjiev. The hybrid nanomaterial PVA/AgNps, as biologically active product with reserved space in the antimicrobial therapy. Letters in Applied NanoBioScience, 2016, 5 (3): 422 – 438.
- Pencheva D., Velichkova E., Sandarov D., Cardoso A., Mileva M., Genova-Kalou P., Bryaskova R. Experimental Research of “Ghost” Escherichia Coli O 104. Journal of Vaccines and Vaccination, 2015, 6 (4), 4 pages.
- Pencheva D., Velichkova E., Sandarov D., Cardoso A., Mileva M., Genova-Kalou P., Bryaskova R. Experimental study on the components in poly-valent "ghost" Salmonella vaccine for veterinary use. Journal of Nanomaterials, 2015, Vol. 2015 (2015), Article ID 101464, 4 pages. (IF = 1.644)
- Radka Argirova, Raina Nenova, Dimitar Ivanov, Petia Genova-Kalou, Sevda Raleva. Experimental model to study co-infection of human immunodeficiency virus – type 1 (HIV-1IIIB) and influenza virus in cell culture. Biotechnology & Biotechnological Equipment, 2015, 6 pages. (IF = 0.300)
- Mileva M., Pencheva D., Bryaskova, R., Genova-Kalou P., Kantardjiev T. Experimental treatment of recurrent otitis externa. Open Veterinary Journal, 2015, 5(1), 38 - 41.
- Genova-Kalou, P., J. Cherneva, M. Jordanova, A. Jordanova. The serological evaluation of the cytomegalovirus (CMV) and Chlamydia pneumoniae infections in patients with cardiovascular diseases (CVDs). J. Sc. & Techn., Medicine, 2013, 3(1), 26 - 31.
- Benedetti F., Berti F., Budal S., Campaner P., Dinon F., Tossi A., Genova P., Atanassov V., Hinkov A. Synthesis and biological activity of potent HIV-1 protease inhibitors based on Phe-Pro dihydroxyethylene isosters. J Med Chem, 2012, 55, 3900 – 3910. (IF = 5.589)
- I. Stankova, K. Stanoeva, A. Hinkov, I. Alexiev, P. Genova-Kalou, R. Chayrov. R. Argirova. Amino acid and peptide esters of abacavir: synthesis and activity against human immunodeficiency virus type 1 in cell culture. Med Chem Res., 2012, 21 (12), 4053 – 4059. (IF = 1.436)
- Genova-Kalou, P., Dundarova, D., Idakieva, K., Mohmmed, A., Dundarov, S., Argirova, R. (2008). Anti-herpes effect of the hemocyanin derived from mollusc Rapana Thomasiana. Z Naturforsch, 63с, р. 429 – 434. (IF = 0.776)
- Kovala-Demertzi, D., Varadinova, T., Genova, P., Souza, Demertzis, M. A. (2007). Platinum(II) and Palladium(II) Complexes of Pyridine-2-carbaldehyde Thiosemicarbazone as Alternative anti-Herpes Simplex Virus Agents. Bioinorg Chem Appl, Published online, Article ID 56165, 1 - 6. (IF = 1.265).
PRICE LIST
Code | Test | Price in BGN |
---|---|---|
103 | Venous blood collection | 6,00 |
311 | Qualitative determination of IgM antibodies by ELISA for HSV-1 | 17,00 |
311 | Qualitative determination of IgG antibodies by ELISA for HSV-1 | 17,00 |
312 | Qualitative determination of IgM antibodies by ELISA for VZV | 17,00 |
312 | Qualitative determination of IgG antibodies by ELISA for VZV | 17,00 |
313 | Qualitative determination of IgM antibodies by ELISA for EBV | 17,00 |
313 | Qualitative determination of IgG antibodies by ELISA for EBV | 17,00 |
314 | Qualitative determination of IgM antibodies by ELISA for CMV | 17,00 |
314 | Qualitative determination of IgG antibodies by ELISA for CMV | 17,00 |
314 | Determination of CMV-IgG antibodies by ELISA / avidity | 17,00 |
360 | Qualitative determination of IgM antibodies by ELISA for HSV-2 | 16,00 |
360 | Qualitative determination of IgG antibodies by ELISA for HSV-2 | 16,00 |
237 | Demonstration of DNA by polymerase chain reaction - qualitative determination of HSV-1 DNA | 41,00 |
237 | Demonstration of DNA by polymerase chain reaction - qualitative determination of HSV-2 DNA | 41,00 |
237 | Demonstration of DNA with polymerase chain reaction - qualitative determination of VZV | 41,00 |
237 | Demonstration of DNA by polymerase chain reaction - qualitative determination of CMV | 41,00 |
237 | Demonstration of DNA by polymerase chain reaction - qualitative determination of EBV DNA | 41,00 |
237 | Demonstration of DNA by polymerase chain reaction - qualitative determination of HHV-6 DNA | 41,00 |
237 | Detection of DNA by polymerase chain reaction - detection of HPV DNA | 41,00 |
319 | Qualitative determination of IgM antibodies by ELISA for Chlamydia trachomatis | 17,00 |
365 | Qualitative determination of IgG antibodies by ELISA for Chlamydia trachomatis | 17,00 |
366 | Qualitative determination of IgA antibodies by ELISA for Chlamydia trachomatis | 17,00 |
364 | ELISA diagnosis of Q fever Phase II IgM antibodies | 17,00 |
364 | ELISA diagnosis of Q fever phase II IgG antibodies | 17,00 |
318 | ELISA diagnosis of Q fever Phase I IgA antibodies | 17,00 |
318 | ELISA diagnosis of Q fever Phase I IgG antibodies | 17,00 |
237 | Demonstration of DNA by polymerase chain reaction - qualitative determination of C. burnetii DNA | 41,00 |
319 | Qualitative determination of IgM antibodies by ELISA for Chlamydia pneumoniae | 17,00 |
365 | Qualitative determination of IgG antibodies by ELISA for Chlamydia pneumoniae | 17,00 |
366 | Qualitative determination of IgA antibodies by ELISA for Chlamydia pneumoniaeChlamydia pneumoniae | 17,00 |
348 | ELISA diagnosis of Mycoplasma pneumoniae IgM antibodies | 17,00 |
349 | ELISA diagnosis of Mycoplasma pneumoniae IgA antibodies | 17,00 |
350 | ELISA diagnosis of Mycoplasma pneumoniae IgG antibodies | 17,00 |
346 | ELISA diagnosis of Marseille fever IgM antibodies | 17,00 |
347 | ELISA diagnosis of Marseille fever IgG antibodies | 17,00 |
237 | Demonstration of DNA by polymerase chain reaction - qualitative determination of R.conorii DNA | 41,00 |
227 | Indirect immunofluorescence test for the detection of IgM antibodies to chlamydia | 28,00 |
227 | Indirect immunofluorescence test for the detection of IgG antibodies to chlamydia | 28,00 |
227 | Indirect immunofluorescence test for the detection of IgM / IgA / IgG antibodies to C.burnetii phase II | 28,00 |
227 | Indirect immunofluorescence test for IgG antibodies to C. burnetii phase II/I | 28,00 |